The discovery of the complement system dates back to the 19th century. It has since developed into a critical clinical entity. As a crucial part of the body’s innate immune response and as a bridge between innate and acquired immunity, it mediates responses to inflammatory triggers through a coordinated sequential enzyme cascade that leads to foreign cell clearance through.
In this piece, we’ll provide a basic rundown of the rabbit and guinea pig complements that are used for research purposes.
Complement System: Basic Functions
These are the primary functions of the complement system:
- Chemotaxis: attracting neutrophils and macrophages
- Opsonization:enhancing antigen phagocytosis
- Agglutination:binding pathogens together
- Cell Lysis:rupturing foreign cell membranes
Here’s a rundown of the rabbit complements that are used for research.
- Standard Complement: Rabbit complement, whether lyophilized or frozen, is sourced from rabbit serum. When using human peripheral blood lymphocytes (PBLs) and HLA-ABC tissue typing for standard lymphocytotoxicity procedures, rabbit complement is used as a source for a complement system.
- Low-Tox-H Complement: When rabbit serum undergoes a process to reduce toxicity with regard to human peripheral blood B-lymphocytes, you get Low-Tox-H Rabbit Complement. This is used as a complement system source for HLA-DR tissue typing procedures and lymphocytotoxicity assays.
- Low-Tox-M Complement: Lyophilized rabbit serum’s potent toxicity to mouse lymphocytes can be decreased considerably through absorption techniques.
- Low-Tox-R Complement: Low-Tox-R Rabbit Complement is the result of adsorption with sheep erythrocytes and agarose to reduce rabbit serum’s non-specific cytotoxicity to rat lymphocytes.
- Rabbit Complement MA: This is typically used as a complement system source for procedures that utilize monoclonal antibodies to human lymphocytes.
- Baby Rabbit Complement: Baby rabbit complements are derived from 3 to 4 weeks-old rabbits and are used in cytotoxicity research. Baby rabbit complement is tested against peripheral lymphocytes by utilizing primary monoclonal antibodies.
Guinea Pig Complement
Here’s a rundown of the guinea pig complements used for research.
- Standard Complement: Standard guinea pig complement is a reconstituted serum that’s used in systems that require a source of its unique properties, such as hemolytic antibody titrations, complement fixation, hemolytic assays.
- Low-Tox Complement: This is a very potent complement source that undergoes adsorption to eliminate background toxicity to lymphocytes and thymocytes, particularly those of mice.
Pel-Freez Biologicals is a leading US supplier of normal rabbit serum, adult rabbit complement, guinea pig complement, IgG/IgM/antibody depleted complement, and more to leading research bodies in the country.